Galectin 2 Recombinant monoclonal antibody

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• Galectin 2 Recombinant monoclonal antibody (87897-1-RR)

• 

  WB analysis of mouse small intestine using 87897-1-RR

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 87897-1-RR (Galectin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsil using 87897-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 87897-1-RR (Galectin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 87897-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87897-1-RR against Human Galectin 2 were performed. The affinity constant is below 1 pM.

Galectin 2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87897-1-RR

Clone No.260048G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Beta-galactoside-binding lectin L-14-II, Gal 2, Gal-2, HL14, Lactose binding lectin 2

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Tested Applications

• Positive WB detected in: mouse small intestine tissue
• Positive IHC detected in: human tonsil tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87897-1-RR targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg7632

  Product name: Recombinant human LGALS2 protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 2-132 aa of BC059782

  Sequence: TGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE

• Full Name: lectin, galactoside-binding, soluble, 2
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC059782
• Gene Symbol: Galectin 2
• Gene ID (NCBI): 3957
• RRID: AB_3745814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05162
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a class of carbohydrate-binding proteins implicated in multiple physiological processes including inflammation, immune responses, cell migration, autophagy and signal transduction (PMID: 29717004). Galectin-2 (Gal-2, also known as LGALS2) is a monocyte-derived carbohydrate-binding lectin; its upregulation correlates with reduced collateral arteriogenesis in patients with obstructive coronary artery disease (PMID: 25884209). Additionally, Gal-2 may interact with T cells, particularly in the setting of inflammatory bowel disease (PMID: 15356130).