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Glutamine Synthetase Monoclonal antibody
Glutamine Synthetase Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1B6G4
Cat no : 66323-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human liver cancer tissue, rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Sample-dependent, check data in validation data gallery |
Product Information
66323-2-Ig targets Glutamine Synthetase in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Glutamine Synthetase fusion protein Ag6309 |
Full Name | glutamate-ammonia ligase (glutamine synthetase) |
Calculated molecular weight | 374 aa, 42 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC011700 |
Gene symbol | GLUL |
Gene ID (NCBI) | 2752 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Glutamine Synthetase antibody 66323-2-Ig | Download protocol |
IHC protocol for Glutamine Synthetase antibody 66323-2-Ig | Download protocol |
FC protocol for Glutamine Synthetase antibody 66323-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |