Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B12G10

Cat no : 66778-1-Ig

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

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Validation Data Gallery

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  WB analysis of human blood using 66778-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66778-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 66778-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human placenta using 66778-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of K-562 using 66778-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Glycophorin A (66778-1-Ig, Clone:2B12G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human blood tissue
• Positive IHC detected in: human placenta tissue, human stomach cancer tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human placenta tissue
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66778-1-Ig targets Glycophorin A in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycophorin A fusion protein Ag8635
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: GYPA
• Gene ID (NCBI): 2993
• RRID: AB_2882124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Publications

Species	Application	Title
human	WB	J Biochem; C-terminally truncation is a prominent post-translational modification of human erythrocyte α-synuclein; Authors - Ryosuke Amagai

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C