Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2B12G10

Cat no : 66778-1-Ig

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha



Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66778-1-Ig targets Glycophorin A in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glycophorin A fusion protein Ag8635
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC005319
Gene symbol GYPA
Gene ID (NCBI) 2993
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
WB protocol for Glycophorin A antibody 66778-1-IgDownload protocol
IHC protocol for Glycophorin A antibody 66778-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols