Glycophorin A Monoclonal antibody
Glycophorin A Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2B12G10
Cat no : 66778-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue |
Positive IHC detected in | human placenta tissue, human stomach cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human placenta tissue |
Positive FC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66778-1-Ig targets Glycophorin A in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Glycophorin A fusion protein Ag8635 |
Full Name | glycophorin A (MNS blood group) |
Calculated molecular weight | 150 aa, 16 kDa |
Observed molecular weight | 36-38 kDa |
GenBank accession number | BC005319 |
Gene symbol | GYPA |
Gene ID (NCBI) | 2993 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
Protocols
Product Specific Protocols | |
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WB protocol for Glycophorin A antibody 66778-1-Ig | Download protocol |
IHC protocol for Glycophorin A antibody 66778-1-Ig | Download protocol |
IF protocol for Glycophorin A antibody 66778-1-Ig | Download protocol |
FC protocol for Glycophorin A antibody 66778-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |