Mouse GPNMB Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis of RAW 264.7 using 86874-1-RR (same clone as 86874-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  WB analysis using 86874-1-RR (same clone as 86874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02737-1

  Serum of six mice was measured. The GPNMB concentration of detected samples was determined to be 14.00 ng/mL with a range of 10.09-19.47 ng/mL.

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  Sandwich ELISA standard curve of MP02737-1

  Sandwich ELISA standard curve of MP02737-1, Mouse GPNMB Recombinant Matched Antibody Pair - PBS only. 86874-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2814. 86874-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

GPNMB Uni-rAb^® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Cat No. 86874-1-PBS

Clone No.250309C11

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Synonyms

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

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Product Information

86874-1-PBS targets GPNMB as part of a matched antibody pair:

MP02737-1: 86874-1-PBS capture and 86874-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2814

  Product name: Recombinant Mouse GPNMB protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 23-502 aa of NM_053110.4

  Sequence: KRFRDVLGHEQYPDHMREHNQLRGWSSDENEWDEHLYPVWRRGDGRWKDSWEGGRVQAVLTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYEKNCRNDLGLTSDLHVYNWTAGADDGDWEDGTSRSQHLRFPDRRPFPRPHGWKKWSFVYVFHTLGQYFQKLGRCSARVSINTVNLTAGPQVMEVTVFRRYGRAYIPISKVKDVYVITDQIPVFVTMSQKNDRNLSDEIFLRDLPIVFDVLIHDPSHFLNDSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNLNLTVQTAVPGPCPPPSPSTPPPPSTPPSPPPSPLPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVEGILEVSIMQIADVPMPTPQPANSLMDFTVTCKGATPMEACTIISDPTCQIAQNRVCSPVAVDGLCLLSVRRAFNGSGTYCVNFTLGDDASLALTSTLISIPGKDPDSPLRAVN

• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 90-100 kDa, 120kDa
• GenBank Accession Number: NM_053110.4
• Gene Symbol: Gpnmb
• Gene ID (NCBI): 93695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99P91
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.