GPNMB Recombinant monoclonal antibody

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  WB analysis of RAW 264.7 using 86874-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86874-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 86874-1-RR

  IP result of anti-GPNMB (IP:86874-1-RR, 4ug; Detection:86874-1-RR 1:1500) with mouse skin tissue lysate 1120 ug.

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  IHC staining of mouse heart using 86874-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 86874-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPNMB Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86874-1-RR

Clone No.250309C11

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse skin tissue, rat skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86874-1-RR targets GPNMB in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2814

  Product name: Recombinant Mouse GPNMB protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 23-502 aa of NM_053110.4

  Sequence: KRFRDVLGHEQYPDHMREHNQLRGWSSDENEWDEHLYPVWRRGDGRWKDSWEGGRVQAVLTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYEKNCRNDLGLTSDLHVYNWTAGADDGDWEDGTSRSQHLRFPDRRPFPRPHGWKKWSFVYVFHTLGQYFQKLGRCSARVSINTVNLTAGPQVMEVTVFRRYGRAYIPISKVKDVYVITDQIPVFVTMSQKNDRNLSDEIFLRDLPIVFDVLIHDPSHFLNDSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNLNLTVQTAVPGPCPPPSPSTPPPPSTPPSPPPSPLPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVEGILEVSIMQIADVPMPTPQPANSLMDFTVTCKGATPMEACTIISDPTCQIAQNRVCSPVAVDGLCLLSVRRAFNGSGTYCVNFTLGDDASLALTSTLISIPGKDPDSPLRAVN

• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 90-100 kDa, 120kDa
• GenBank Accession Number: NM_053110.4
• Gene Symbol: Gpnmb
• Gene ID (NCBI): 93695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99P91
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.