CoraLite® Plus 488-conjugated Granzyme A Recombinant antibody

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  IF Staining of Jurkat using CL488-84583-3

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 Granzyme A antibody (CL488-84583-3, Clone: 241979B8 ) at dilution of 1:200.

Granzyme A Recombinant Antibody for IF/ICC

Cat No. CL488-84583-3

Clone No.241979B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

GrA, GZMA, 241979B8, CTL tryptase, CTLA3

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84583-3 targets Granzyme A in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Granzyme A fusion protein Ag1818
• Full Name: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC015739
• Gene Symbol: Granzyme A
• Gene ID (NCBI): 3001
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12544
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.