Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, A431 cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | skin cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive ChIP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Chromatin immunoprecipitation (ChIP) | CHIP : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17510-1-AP targets Histone H1.0 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9982 Product name: Recombinant human Histone H1.0 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC000145 Sequence: MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK Predict reactive species |
| Full Name | H1 histone family, member 0 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC000145 |
| Gene Symbol | Histone H1.0 |
| Gene ID (NCBI) | 3005 |
| RRID | AB_10695628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Histone H1.0 antibody 17510-1-AP | Download protocol |
| IHC protocol for Histone H1.0 antibody 17510-1-AP | Download protocol |
| IP protocol for Histone H1.0 antibody 17510-1-AP | Download protocol |
| WB protocol for Histone H1.0 antibody 17510-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Histone H1.0 antibody (Cat# 17510-1-AP) has 19 total citations published in journals including Nature Neuroscience, Molecular Cell, Nucleic Acids Research, Nature Cardiovascular Research, Oncogene, Theranostics, Cell Death Discovery, PLoS Pathogens, Inflammation, Cell Signaling, Scientific Reports, Cell Cycle, and J Cancer. Associated research fields encompass chromatin biology, epigenetic regulation, cancer (prostate, bladder, pancreatic, hepatocellular), viral infection, DNA repair, ferroptosis, cardiac mechanics, renal fibrosis, airway inflammation, and neuronal glucose metabolism.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage | ||
Mol Cell CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor tolerance | ||
Nucleic Acids Res The dual ubiquitin binding mode of SPRTN secures rapid spatiotemporal proteolysis of DNA-protein crosslinks | ||
Nucleic Acids Res Pin1 promotes GR transactivation by enhancing recruitment to target genes. | ||
