Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse heart tissue, Raji cells, HL-60 cells, HEK-293 ells, mouse kidney, rat kidney|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells, U-251 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10856-1-AP targets Histone H2A.X in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone H2A.X fusion protein Ag1305|
|Full Name||H2A histone family, member X|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15-18 kDa|
|GenBank accession number||BC013416|
|Gene ID (NCBI)||3014|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.
|Product Specific Protocols|
|WB protocol for Histone H2A.X antibody 10856-1-AP||Download protocol|
|IHC protocol for Histone H2A.X antibody 10856-1-AP||Download protocol|
|IF protocol for Histone H2A.X antibody 10856-1-AP||Download protocol|
|FC protocol for Histone H2A.X antibody 10856-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andreas (Verified Customer) (03-23-2022)
Antibody gives very good signal, hence the dilution factor. One important note is that I include 1:2000 dilution of benzonase in my lysis buffer to recover most of the chromatin-bound material, which big part of it is otherwise lost during the clarification step.
kes (Verified Customer) (01-17-2022)
used for WB (1:1000) in milk+TBST buffer and got good results