HAUS1 Polyclonal antibody, PBS Only

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• HAUS1 Polyclonal antibody, PBS Only (11094-2-PBS)

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  WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 11094-2-AP (same clone as 11094-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

HAUS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 11094-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CCDC5, Coiled-coil domain-containing protein 5, Enhancer of invasion cluster, Enhancer of invasion-cluster, HAUS augmin-like complex subunit 1

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Product Information

11094-2-PBS targets HAUS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1569

  Product name: Recombinant human CCDC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-278 aa of BC014003

  Sequence: MEPQEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAKYLQDLLMESVNFSPANLSSTGSRYLNALVDSAVALETKDTSLASFIPAVNDLTSDLFRTKSKSEEIKIELEKLEKNLTATLVLEKCLQEDVKKAELHLSTERAKVDNRRQNMDFLKAKSEEFRFGIKAAEEQLSARGMDASLSHQSLVALSEKLARLKQQTIPLKKKLESYLDLMPNPSLAQVKIEEAKRELDSIEAELTRRVDMMEL

• Full Name: coiled-coil domain containing 5 (spindle associated)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014003
• Gene Symbol: HAUS1
• Gene ID (NCBI): 115106
• RRID: AB_614986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CS2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.