TIM3 Monoclonal antibody

TIM3 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, pig, mouse, rat







Cat no : 60355-1-Ig


CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

Tested Applications

Positive WB detected inDaudi cells, PC-3 cells, pig thymus tissue, ROS1728 cells, human heart tissue, RAW 264.7 cells, rat heart tissue
Positive FC detected inDaudi cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

60355-1-Ig targets TIM3 in WB, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIM3 fusion protein Ag16901
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 33 kDa, 50-70 kDa
GenBank accession numberBC020843
Gene symbol HAVCR2
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).


Product Specific Protocols
WB protocol for TIM3 antibody 60355-1-IgDownload protocol
FC protocol for TIM3 antibody 60355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes

Authors - Fan Wu


Tumor cell-intrinsic Tim-3 promotes liver cancer via NF-κB/IL-6/STAT3 axis.

Authors - Hualin Zhang

J Pathol

Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.

Authors - Fan Wu

Front Cell Dev Biol

Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.

Authors - Lin Liang

Front Immunol

Up-Regulation of Immune Checkpoints in the Thymus of PRRSV-1-Infected Piglets in a Virulence-Dependent Fashion.

Authors - Inés Ruedas-Torres

Front Immunol

Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.

Authors - Guanzhang Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Santhosh (Verified Customer) (04-15-2019)

Human Tonsil FFPE tissue. Heat mediated antigen retrieval (pH 6)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
TIM3 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:60355-1-Ig)