HAX1 Polyclonal antibody, PBS Only

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• HAX1 Polyclonal antibody, PBS Only (11266-1-PBS)

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  WB analysis of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11266-1-AP (same clone as 11266-1-PBS)

  WB result of HAX1 antibody (11266-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11266-1-AP (same clone as 11266-1-PBS)

  IP result of anti-HAX1 (IP:11266-1-AP, 3ug; Detection:11266-1-AP 1:300) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAX1 antibody (11266-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HAX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11266-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HAX 1, HAX-1, HCLS1-associated protein X-1, HCLSBP1, HS1-associating protein X-1

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Product Information

11266-1-PBS targets HAX1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1792

  Product name: Recombinant human HAX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-279 aa of BC014314

  Sequence: MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR

• Full Name: HCLS1 associated protein X-1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014314
• Gene Symbol: HAX1
• Gene ID (NCBI): 10456
• RRID: AB_2263720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00165
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.