HBS1L Recombinant monoclonal antibody

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  WB analysis using 82795-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82795-2-RR (HBS1L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82795-2-RR

  WB result of HBS1L antibody (82795-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 82795-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82795-2-RR against Human HBS1L were performed. The affinity constant is 0.931 nM.

• Featured Product
• KD/KO Validated

HBS1L Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82795-2-RR

Clone No.2L24

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82795-2-RR targets HBS1L in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0388

  Product name: Recombinant human HBS1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 43-262 aa of BC001465

  Sequence: SRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPGVSSEENGHSFHTPQKGPPIEDAIASSDVLETASKSANPPHTIQASEEQSSTPAPVKKSGKLRQQIDVKAELEKRQGGKQLLN

• Full Name: HBS1-like (S. cerevisiae)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC001465
• Gene Symbol: HBS1L
• Gene ID (NCBI): 10767
• RRID: AB_3086540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y450
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.