HCN4 Recombinant antibody

• 

  WB analysis using 85222-4-RR

  Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 85222-4-RR (HCN4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HCN4 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85222-4-RR

Clone No.242695D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, 242695D11

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85222-4-RR targets HCN4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_005477
• Gene Symbol: HCN4
• Gene ID (NCBI): 10021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y3Q4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN4 belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP. HCN4 may mediate responses to sour stimuli. (PMID:10228147). Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2). Defects in HCN4 are the cause of Brugada syndrome type 8 (BRS8).