HDAC9 Monoclonal antibody

HDAC9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1G6C5

Cat no : 67364-1-Ig

Synonyms

DKFZp779K1053, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR

---

Validation Data Gallery

• 

  WB analysis using 67364-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67364-1-Ig (HDAC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67364-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67364-1-Ig (HDAC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, Daudi cells, HepG2 cells, Raji cells, K-562 cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67364-1-Ig targets HDAC9 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC9 fusion protein Ag28514
• Full Name: histone deacetylase 9
• Calculated Molecular Weight: 1011 aa, 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC152405
• Gene Symbol: HDAC9
• Gene ID (NCBI): 9734
• RRID: AB_2882616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle and testis.

Publications

Species	Application	Title
human	WB	Int J Ophthalmol; Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism; Authors - Jia-Yi Ning
human	WB	Cell Death Dis; WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.; Authors - Yingtong Feng