HDGF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HDGF2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 15134-1-AP

Synonyms

HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2

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Validation Data Gallery

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

15134-1-AP targets HDGF2 in IHC, IF/ICC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDGF2 fusion protein Ag7281
• Full Name: hepatoma-derived growth factor-related protein 2
• Calculated Molecular Weight: 74 kDa
• GenBank Accession Number: BC000755
• Gene Symbol: HDGF2
• Gene ID (NCBI): 84717
• RRID: AB_2117220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD; Authors - Sahba Seddighi
human	WB	Sci Adv; The H3K36me2 writer-reader dependency in H3K27M-DIPG.; Authors - Jia-Ray Yu ; KD Validated
human	WB	Sci Adv; LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.; Authors - Gary LeRoy
mouse	WB,chIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu ; KD Validated
human	IF, IP	Nucleic Acids Res; Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.; Authors - Annika Baude
human	WB	Cell Rep; A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.; Authors - Judd F Hultquist ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Works in ICC (nuclear staining) and WB (no non specific bands)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

HDGF2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:15134-1-AP)