Tested Applications
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15134-1-AP targets HDGF2 in IHC, IF/ICC, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7281 Product name: Recombinant human HDGF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 321-670 aa of BC000755 Sequence: EARRRELEARRRREQEEELRRLREQEKEEKERRRERADRGEAERGSGGSSGDELREDDEPVKKRGRKGRGRGPPSSSDSEPEAELEREAKKSAKKPQSSSTEPARKPGQKEKRVRPEEKQQAKPVKVERTRKRSEGFSMDRKVEKKKEPSVEEKLQKLHSEIKFALKVDSPDVKRCLNALEELGTLQVTSQILQKNTDVVATLKKIRRYKANKDVMEKAAEVYTRLKSRVLGPKIEAVQKVNKAGMEKEKAEEKLAGEELAGEEAPQEKAEDKPSTDLSAPVNGEATSQKGESAEDKEHEEGRDSEEGPRCGSSEDLHDVREGPDLDRPGSDRQERERARGDSEALDEES Predict reactive species |
| Full Name | hepatoma-derived growth factor-related protein 2 |
| Calculated Molecular Weight | 74 kDa |
| GenBank Accession Number | BC000755 |
| Gene Symbol | HDGF2 |
| Gene ID (NCBI) | 84717 |
| RRID | AB_2117220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z4V5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDGF2 antibody 15134-1-AP | Download protocol |
| IHC protocol for HDGF2 antibody 15134-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HDGF2 Polyclonal antibody (Cat# 15134-1-AP) has 17 citations published in journals including Science Translational Medicine, Nucleic Acids Research, Science Advances, Cell Reports, Movement Disorders, and Oncology Letters. Associated research fields encompass neurodegeneration (ALS/FTD, TDP-43 proteinopathies), epigenetics and chromatin remodeling, DNA repair, HIV-host interactions, cardiac electrophysiology, traumatic brain injury, multiple cancer types (pancreatic, myeloma, osteosarcoma, lung), preeclampsia, and apoptosis.
| Species | Application | Title |
|---|---|---|
Sci Transl Med Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD | ||
Nucleic Acids Res Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. | ||
Nucleic Acids Res HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.
| ||
Sci Adv M6A-dependent RNA condensation underlies FUS autoregulation and can be harnessed for ALS therapy development | ||
Sci Adv LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells. |
Reviews
What customers say
Both reviewers report positive results. One user (4/5) used the antibody at 1:1000 for Western Blot on liver tissue and found it performed well. The other (5/5) used it at 1:1000 for both immunofluorescence (nuclear staining) and Western Blot on SH-SY5Y cells, noting clean nuclear staining and no non-specific bands. Overall, the antibody is well-received for WB and IF applications with consistent specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-26-2025) | GOOD FOR WB
|
Tatyana (Verified Customer) (12-04-2023) | Works in ICC (nuclear staining) and WB (no non specific bands)
 |
