HELB Polyclonal antibody

HELB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24700-1-AP

Synonyms

hDHB, EC:3.6.4.12

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Validation Data Gallery

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  WB analysis of Jurkat using 24700-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24700-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24700-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24700-1-AP

  IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IF Staining of HeLa using 24700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HELB antibody (24700-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24700-1-AP targets HELB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HELB fusion protein Ag20335
• Full Name: helicase (DNA) B
• Calculated Molecular Weight: 1087 aa, 123 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC157019
• Gene Symbol: HELB
• Gene ID (NCBI): 92797
• RRID: AB_2879680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.