HERPUD1 Recombinant antibody, PBS Only

• 

  WB analysis of BxPC-3 using 85627-1-RR (same clone as 85627-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 85627-1-RR (same clone as 85627-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 85627-1-RR (same clone as 85627-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

HERPUD1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85627-1-PBS

Clone No.243054E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

---

Product Information

85627-1-PBS targets HERPUD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HERPUD1 fusion protein Ag34891
• Full Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC008320
• Gene Symbol: HERPUD1
• Gene ID (NCBI): 9709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15011
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).