HERPUD1 Recombinant antibody

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  WB analysis of BxPC-3 using 85627-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85627-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 85627-1-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HERPUD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85627-1-RR

Clone No.243054E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

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Tested Applications

• Positive WB detected in: LNCaP cells, BxPC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85627-1-RR targets HERPUD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HERPUD1 fusion protein Ag34891
• Full Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC008320
• Gene Symbol: HERPUD1
• Gene ID (NCBI): 9709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15011
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).