HEXA Monoclonal antibody

HEXA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A4F2

Cat no : 60337-1-Ig

Synonyms

HEXA, Hexosaminidase subunit A, TSD

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Validation Data Gallery

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  WB analysis using 60337-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of A431 using 60337-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit testis using 60337-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, rabbit testis tissue, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60337-1-Ig targets HEXA in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HEXA fusion protein Ag1854
• Full Name: hexosaminidase A (alpha polypeptide)
• Calculated Molecular Weight: 529 aa, 60 kDa
• Observed Molecular Weight: 50-54 kDa, 60-67 kDa
• GenBank Accession Number: BC018927
• Gene Symbol: HEXA
• Gene ID (NCBI): 3073
• RRID: AB_2881446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).