HEXB Polyclonal antibody, PBS Only

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• HEXB Polyclonal antibody, PBS Only (16229-1-PBS)

• 

  WB analysis using 16229-1-AP (same clone as 16229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16229-1-AP (same clone as 16229-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16229-1-AP (same clone as 16229-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16229-1-AP (same clone as 16229-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 16229-1-AP (same clone as 16229-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 16229-1-AP (same clone as 16229-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HEXB Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16229-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16229-1-PBS targets HEXB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8683

  Product name: Recombinant human HEXB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-332 aa of BC017378

  Sequence: MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSF

• Full Name: hexosaminidase B (beta polypeptide)
• Calculated Molecular Weight: 556 aa, 63 kDa
• Observed Molecular Weight: 63-67 kDa
• GenBank Accession Number: BC017378
• Gene Symbol: HEXB
• Gene ID (NCBI): 3074
• RRID: AB_2264075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07686
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).