HFE Polyclonal antibody, PBS Only

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• HFE Polyclonal antibody, PBS Only (17954-1-PBS)

• 

  WB analysis using 17954-1-AP (same clone as 17954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17954-1-AP (HFE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17954-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 17954-1-AP (same clone as 17954-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 17954-1-AP (HFE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17954-1-PBS in a different storage buffer formulation.

HFE Polyclonal Antibody for WB, Indirect ELISA

Cat No. 17954-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

hemochromatosis, HFE1, HH, HLA H, HLAH

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Product Information

17954-1-PBS targets HFE in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12448

  Product name: Recombinant human HFE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-295 aa of BC074721

  Sequence: SLHYLFMGASEQDLGLSLFEALGYVDDQLFVFYDHESRRVEPRTPWVSSRISSQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSKESHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQEGSESSSLPGKVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWE

• Full Name: hemochromatosis
• Calculated Molecular Weight: 345 aa, 40 kDa
• Observed Molecular Weight: 45-58 kDa
• GenBank Accession Number: BC074721
• Gene Symbol: HFE
• Gene ID (NCBI): 3077
• RRID: AB_10596779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q30201
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HFE is an important iron metabolism regulatory protein, which regulates the production of hepcidin through interactions with other proteins such as transferrin receptor 1 (TfR1) and TfR2. HFE is the most common pathogenic gene for hereditary hemochromatosis, which is a hereditary disease of iron accumulation in multiple organs caused by mutations in genes regulating iron metabolism.