HGD Recombinant antibody

HGD Recombinant Antibody for WB, IHC, ELISA

Cat No. 84931-5-RR
Clone No.242485E11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

242485E11, AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84931-5-RR targets HGD in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HGD fusion protein Ag9544 Predict reactive species
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ93099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

Protocols

Product Specific Protocols
WB protocol for HGD antibody 84931-5-RRDownload protocol
IHC protocol for HGD antibody 84931-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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