HGS Monoclonal antibody

HGS Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3B10D6

Cat no : 67818-1-Ig

Synonyms

HGS, HRS, Protein pp110, ZFYVE8



Tested Applications

Positive WB detected inLNCaP cells, PC-12 cells, Neuro-2a cells, Jurkat cells, A549 cells, HeLa cells, HEK-293 cells, K-562 cells, rat brain tissue, mouse brain tissue, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67818-1-Ig targets HGS in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HGS fusion protein Ag28610
Full Name hepatocyte growth factor-regulated tyrosine kinase substrate
Calculated molecular weight 86 kDa
Observed molecular weight110 kDa
GenBank accession numberBC003565
Gene symbol HGS
Gene ID (NCBI) 9146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Protocols

Product Specific Protocols
WB protocol for HGS antibody 67818-1-IgDownload protocol
IHC protocol for HGS antibody 67818-1-IgDownload protocol
IF protocol for HGS antibody 67818-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols