HIP1 Polyclonal antibody, PBS Only

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• HIP1 Polyclonal antibody, PBS Only (22231-1-PBS)

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  WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 22231-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

  IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22231-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

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Product Information

22231-1-PBS targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17576

  Product name: Recombinant human HIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 435-764 aa of BC110545

  Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG

• Full Name: huntingtin interacting protein 1
• Calculated Molecular Weight: 1037 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC110545
• Gene Symbol: HIP1
• Gene ID (NCBI): 3092
• RRID: AB_2879041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O00291
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.