HIP55 Polyclonal antibody, PBS Only

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• HIP55 Polyclonal antibody, PBS Only (13015-1-PBS)

• 

  WB analysis of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 13015-1-AP (same clone as 13015-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13015-1-AP (same clone as 13015-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13015-1-AP (same clone as 13015-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13015-1-AP (same clone as 13015-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 13015-1-AP (same clone as 13015-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

  IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using DBNL antibody 13015-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HIP55 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 13015-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

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Product Information

13015-1-PBS targets HIP55 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4078

  Product name: Recombinant human DBNL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 131-430 aa of BC031687

  Sequence: KVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE

• Full Name: drebrin-like
• Calculated Molecular Weight: 430 aa, 48 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC031687
• Gene Symbol: HIP55
• Gene ID (NCBI): 28988
• RRID: AB_2230306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJU6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.