HIPK3 Polyclonal antibody

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  WB analysis using 25107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25107-1-AP (HIPK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25107-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25107-1-AP (HIPK3 Antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human testis using 25107-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25107-1-AP (HIPK3 Antibody) at dilution of 1:2000 (under 40x lens).

HIPK3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25107-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ANPK, DYRK6, FIST, FIST3, HIPK3, Homolog of protein kinase YAK1, PKY, YAK1

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

25107-1-AP targets HIPK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18897

  Product name: Recombinant human HIPK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 884-1039 aa of BC156873

  Sequence: RHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGT

• Full Name: homeodomain interacting protein kinase 3
• Calculated Molecular Weight: 1215 aa, 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC156873
• Gene Symbol: HIPK3
• Gene ID (NCBI): 10114
• RRID: AB_2879899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H422
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Aging (Albany NY); Identification of HIPK3 as a potential biomarker and an inhibitor of clear cell renal cell carcinoma.; Authors - Wen Xiao
human	WB,IHC	Sci Rep; Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3; Authors - Aye Myat Mon