HIPK3 Polyclonal antibody

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• HIPK3 Polyclonal antibody (25107-1-AP)

HIPK3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25107-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ANPK, DYRK6, FIST, FIST3, HIPK3, Homolog of protein kinase YAK1, PKY, YAK1

• 

  WB analysis using 25107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25107-1-AP (HIPK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25107-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25107-1-AP (HIPK3 Antibody) at dilution of 1:2000 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25107-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25107-1-AP (HIPK3 Antibody) at dilution of 1:2000 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25107-1-AP targets HIPK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18897

  Product name: Recombinant human HIPK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 884-1039 aa of BC156873

  Sequence: RHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGT

• Full Name: homeodomain interacting protein kinase 3
• Calculated Molecular Weight: 1215 aa, 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC156873
• Gene Symbol: HIPK3
• Gene ID (NCBI): 10114
• RRID: AB_2879899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H422
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-HIPK3 antibody (Cat# 25107-1-AP) has 2 total citations. These appear in Aging (Albany NY) and Scientific Reports. Both studies used human samples with WB and IHC applications. The associated research fields include clear cell renal cell carcinoma biomarker identification and cholangiocarcinoma growth regulation through the microRNA-205-5p/HIPK3 signaling axis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Aging (Albany NY); Identification of HIPK3 as a potential biomarker and an inhibitor of clear cell renal cell carcinoma.; Authors - Wen Xiao
human	WB,IHC	Sci Rep; Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3; Authors - Aye Myat Mon