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Histone H4 Polyclonal antibody
Histone H4 Polyclonal Antibody for ChIP, IF, IHC, IP, WB, ELISA
Cat no : 16047-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, rat thymus tissue, HepG2 cells, mouse kidney tissue, mouse thymus tissue, MCF-7 cells, HT-1080 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse small intestine tissue, mouse colon tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive ChIP detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|CHImmunoprecipitation (IP)||CHIP : |
|Sample-dependent, check data in validation data gallery|
16047-1-AP targets Histone H4 in WB, RIP, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, pig, yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone H4 fusion protein Ag8999|
|Full Name||histone cluster 1, H4e|
|Calculated molecular weight||102 aa, 11 kDa|
|Observed molecular weight||14 kDa, 11 kDa|
|GenBank accession number||BC012587|
|Gene ID (NCBI)||8367|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
|Product Specific Protocols|
|WB protocol for Histone H4 antibody 16047-1-AP||Download protocol|
|IHC protocol for Histone H4 antibody 16047-1-AP||Download protocol|
|IF protocol for Histone H4 antibody 16047-1-AP||Download protocol|
|IP protocol for Histone H4 antibody 16047-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.
Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.
SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.
Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.
The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.
Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.