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  • KD/KO Validated

Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for ChIP, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, RIP, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16047-1-AP

Synonyms

H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/j, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4, histone, histone cluster 1, H4e, Histone H4, histoneh4



Tested Applications

Positive WB detected inHeLa cells, rat thymus tissue, HepG2 cells, mouse kidney tissue, mouse thymus tissue, MCF-7 cells, HT-1080 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue, mouse colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive ChIP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:20-1:200
CHImmunoprecipitation (IP)CHIP :
Sample-dependent, check data in validation data gallery

Product Information

16047-1-AP targets Histone H4 in WB, RIP, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, pig, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H4 fusion protein Ag8999
Full Name histone cluster 1, H4e
Calculated molecular weight 102 aa, 11 kDa
Observed molecular weight 14 kDa, 11 kDa
GenBank accession numberBC012587
Gene symbol HIST1H4E
Gene ID (NCBI) 8367
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protocols

Product Specific Protocols
WB protocol for Histone H4 antibody 16047-1-APDownload protocol
IHC protocol for Histone H4 antibody 16047-1-APDownload protocol
IF protocol for Histone H4 antibody 16047-1-APDownload protocol
IP protocol for Histone H4 antibody 16047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.

Authors - Lilien N Voong
  • KD Validated
humanWB

Cell Res

Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.

Authors - Xianteng Wang
mouseWB

Cell Res

SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.

Authors - Fang Yu
ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
humanWB

Autophagy

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Authors - Liangde Zheng
human,mouseIHC

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans