HK3 Monoclonal antibody, PBS Only

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  WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

HK3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67803-1-PBS

Clone No.3H3C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3

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Product Information

67803-1-PBS targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30798

  Product name: Recombinant human HK3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 622-923 aa of BC028129

  Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

• Full Name: hexokinase 3 (white cell)
• Calculated Molecular Weight: 923 aa, 99 kDa
• Observed Molecular Weight: 99 kDa
• GenBank Accession Number: BC028129
• Gene Symbol: HK3
• Gene ID (NCBI): 3101
• RRID: AB_2918566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P52790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.