Tested Applications
| Positive WB detected in | Raji cells, Ramos cells, RAW 264.7 cells, human saliva, rat spleen tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67803-1-Ig targets HK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30798 Product name: Recombinant human HK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 622-923 aa of BC028129 Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV Predict reactive species |
| Full Name | hexokinase 3 (white cell) |
| Calculated Molecular Weight | 923 aa, 99 kDa |
| Observed Molecular Weight | 99 kDa |
| GenBank Accession Number | BC028129 |
| Gene Symbol | HK3 |
| Gene ID (NCBI) | 3101 |
| RRID | AB_2918566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HK3 antibody 67803-1-Ig | Download protocol |
| IHC protocol for HK3 antibody 67803-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-HK3 antibody (Cat# 67803-1-Ig) has 10 total citations across journals including Burns Trauma, J Headache Pain, Cancer Immunol Immunother, Clin Epigenetics, J Lipid Res, Gene, Biochem Biophys Res Commun, Cytotechnology, and Eur J Histochem. Research fields span sepsis-induced acute lung injury, neuropathic pain, tumor immunology (neuroblastoma, ovarian cancer, hepatocellular carcinoma), glycolysis/metabolic reprogramming, histone lactylation, and microglial lipid metabolism. Applications are predominantly WB and IHC in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Burns Trauma Histone lactylation exacerbates acute lung injury in septic mice by promoting ferroptosis in pulmonary microvascular endothelial cells. | ||
J Headache Pain Effects of FGF4/HIF-1α axis-mediated neuronal glycolysis on neuropathic pain. | ||
Cancer Immunol Immunother Dual roles of HK3 in regulating the network between tumor cells and tumor-associated macrophages in neuroblastoma.
| ||
Clin Epigenetics Hexokinase 3 promoted cytokine production of monocytes by targeting metabolic reprogramming and histone lactylation in sepsis.
| ||
J Lipid Res Hexokinase 2 Promotes ISGylation of Acyl-CoA Synthetase Long-chain Family Member 4 in Sepsis-Induced Microglia Cells | ||
Gene Hirudin suppresses ovarian cancer cell proliferation and glycolysis through inhibiting the NF-κB/HK3 axis. |
