HLA class I (HLA-C) Polyclonal antibody
HLA class I (HLA-C) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15777-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
15777-1-AP targets HLA class I (HLA-C) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HLA class I (HLA-C) fusion protein Ag8461 |
Full Name | major histocompatibility complex, class I, C |
Calculated molecular weight | 366 aa, 41 kDa |
Observed molecular weight | 44 kDa |
GenBank accession number | BC007814 |
Gene symbol | HLA-C |
Gene ID (NCBI) | 3107 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)
Protocols
Product Specific Protocols | |
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WB protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
IHC protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
IF protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
IP protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Matern Fetal Neonatal Med HLA-C promotes proliferation and cell cycle progression in trophoblast cells |