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HLA class I (HLA-C) Polyclonal antibody

HLA class I (HLA-C) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15777-1-AP

Synonyms

D6S204, HLA C, HLA class I (HLA-C), HLA Cw, HLA Cw12, HLA JY3, HLAC, HLA-C, HLC C, MHC class I antigen Cw*7, PSORS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    WB analysis using 15777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IP experiment of Jurkat using 15777-1-AP

    IP Result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IF Staining of HeLa using 15777-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15777-1-AP targets HLA class I (HLA-C) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I (HLA-C) fusion protein Ag8461
Full Name major histocompatibility complex, class I, C
Calculated molecular weight 366 aa, 41 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC007814
Gene symbol HLA-C
Gene ID (NCBI) 3107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IHC protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IF protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IP protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Matern Fetal Neonatal Med

HLA-C promotes proliferation and cell cycle progression in trophoblast cells

Authors - Hong Lv
View Article