HLA class I (HLA-C) Polyclonal antibody

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  WB analysis using 15777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15777-1-AP

  IP result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15777-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HLA class I (HLA-C) antibody (15777-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

HLA class I (HLA-C) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15777-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Synonyms

HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15777-1-AP targets HLA class I (HLA-C) in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA class I (HLA-C) fusion protein Ag8461
• Full Name: major histocompatibility complex, class I, C
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC007814
• Gene Symbol: HLA class I (HLA-C)
• Gene ID (NCBI): 3107
• RRID: AB_1639892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10321
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	WB	J Matern Fetal Neonatal Med; HLA-C promotes proliferation and cell cycle progression in trophoblast cells; Authors - Hong Lv
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang
human	Cell treatment	Nature; Shifts in receptors during submergence of an encephalitic arbovirus; Authors - Wanyu Li