Tested Applications
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16918-1-AP targets HLA-DQA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10224 Product name: Recombinant human HLA-DQA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-220 aa of BC008585 Sequence: EDIVADHVASYGVNLYQSYGPSGQYTHEFDGDEQFYVDLGRKETVWCLPVLRQFRFDPQFALTNIAVLKHNLNSLIKRSNSTAATNEVPEVTVFSKSPVTLGQPNILICLVDNIFPPVVNITWLSNGHSVTEGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSELTETVVC Predict reactive species |
| Full Name | major histocompatibility complex, class II, DQ alpha 1 |
| Calculated Molecular Weight | 254 aa, 28 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC008585 |
| Gene Symbol | HLA-DQA1 |
| Gene ID (NCBI) | 3117 |
| RRID | AB_2878332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01909 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| IHC protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| WB protocol for HLA-DQA1 antibody 16918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HLA-DQA1 Polyclonal antibody (Cat# 16918-1-AP) has 5 total citations published in Life Sciences, iScience, Italian Journal of Pediatrics, Frontiers in Genetics, and PLoS One. Associated research fields include COPD proteomics, breast cancer liver metastasis, pediatric nephrotic syndrome, fetal growth restriction with maternal immune dysregulation, and esophageal squamous cell carcinoma risk genetics.
| Species | Application | Title |
|---|---|---|
Life Sci Identification of proteomic signatures associated with COPD frequent exacerbators. | ||
iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer | ||
Ital J Pediatr Amino acid variants in the HLA-DQA1 and HLA-DQB1 molecules explain the major association of variants with relapse status in pediatric patients with steroid-sensitive nephrotic syndrome | ||
Front Genet Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System. | ||
PLoS One Variations in the MHC region confer risk to esophageal squamous cell carcinoma on the subjects from high-incidence area in northern china. |
