FcZero-rAb™ Biotin Anti-Human HLA-DR (L243) Rabbit IgG Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-FcA65560

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD14, and 0.25 ug Biotin Anti-Human HLA-DR (L243) Rabbit IgG RecAb (Biotin-FcA65560, Clone: L243) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-FcA65560

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human HLA-DR (L243) Rabbit IgG RecAb (Biotin-FcA65560, Clone: L243) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

HLA-DR FcZero-rAb^TM Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA65560

Clone No.L243

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65560 targets HLA-DR in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human lymphoblastoid B-cell line RPMI 8866

• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).