HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 15862-1-AP

Synonyms

DR4, DRB1, DW2.2/DR2.2, HLA DR1B, HLA DRB, HLA DRB1, HLA DRB1*, HLA DRB2, HLA-DRB1, MHC class II antigen DRB1*15, SS1



Tested Applications

Positive WB detected inRaji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15862-1-AP targets HLA-DRB1 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB1 fusion protein Ag8405
Full Name major histocompatibility complex, class II, DR beta 1
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC007920
Gene symbol HLA-DRB1
Gene ID (NCBI) 3123
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IHC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IP protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
FC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Authors - Semini Geo G