Anti-Human HLA class I (HLA-ABC) (W6/32) Mouse IgG2a Recombinant Antibody

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• Anti-Human HLA class I (HLA-ABC) (W6/32) Mouse IgG2a Recombinant Antibody (65693-1-MR)

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  FC experiment of human peripheral blood leukocytes using 65693-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HLA-ABC (W6/32) Mouse IgG2a RecAb (65693-1-MR, Clone: W6/32) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 65693-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HLA-ABC (W6/32) Mouse IgG2a RecAb (65693-1-MR, Clone: W6/32) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

HLA class I (HLA-ABC) Uni-rAb^® Recombinant Antibody for FC

Cat No. 65693-1-MR

Clone No.W6/32

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

HLA-A, HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65693-1-MR targets HLA class I (HLA-ABC) in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: major histocompatibility complex, class I, A
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: BC003069
• Gene Symbol: HLA-A
• Gene ID (NCBI): 3105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04439
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)