HLX Polyclonal antibody, PBS Only

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• HLX Polyclonal antibody, PBS Only (14336-1-PBS)

• 

  WB analysis of human placenta using 14336-1-AP (same clone as 14336-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14336-1-AP (HLX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14336-1-AP (same clone as 14336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14336-1-AP (same clone as 14336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 14336-1-AP (same clone as 14336-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14336-1-AP (HLX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HLX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14336-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

14336-1-PBS targets HLX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5627

  Product name: Recombinant human HLX protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 134-488 aa of BC033808

  Sequence: QQQQPPPPPRAGALQPPASGTRVVPNPHHSGSAPAPSSKDLKFGIDRILSAEFDPKVKEGNTLRDLTSLLTGGRPAGVHLSGLQPSAGQFFASLDPINEASAILSPLNSNPRNSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSRAVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKDKEAGEKPSGGAPAADGEQDERSPSRSEGEAESESSDSESLDMAPSDTERTEGSERSLHQTTVIKAPVTGALITASSAGSGGSSGGGGNSFSFSSASSLSSSSTSAGCASSLGGGGASELLPATQPTASSAPKSPEPAQGALGCL

• Full Name: H2.0-like homeobox
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC033808
• Gene Symbol: HLX
• Gene ID (NCBI): 3142
• RRID: AB_2232971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14774
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184].