SPP Polyclonal antibody

• Featured Product
• KD/KO Validated

SPP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20416-1-AP

Synonyms

H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP

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Validation Data Gallery

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  WB analysis of HEK-293 using 20416-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20416-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 20416-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20416-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, mouse kidney tissue, U2OS cells
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 20416-1-AP is SPP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPP fusion protein Ag14158
• Full Name: histocompatibility (minor) 13
• Calculated Molecular Weight: 377 aa, 41 kDa
• Observed Molecular Weight: 45 kDa, 100 kDa
• GenBank Accession Number: BC008938
• Gene Symbol: HM13/SPP
• Gene ID (NCBI): 81502
• RRID: AB_10665421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		PLoS One; Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase.; Authors - Schedin-Weiss Sophia S
human	IHC,WB	Cell Death Dis; Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway; Authors - Haiyan Yang ; KD Validated
human	WB	Front Pharmacol; Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma; Authors - Jun Liu ; KD Validated