HMGCL Polyclonal antibody

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  WB analysis using 16898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16898-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16898-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 16898-1-AP

  IP result of anti-HMGCL (IP:16898-1-AP, 4ug; Detection:16898-1-AP 1:500) with mouse spleen tissue lysate 4000 ug.

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  IHC staining of human liver using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16898-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human liver tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10426

  Product name: Recombinant human HMGCL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC010570

  Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

• Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Calculated Molecular Weight: 325 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC010570
• Gene Symbol: HMGCL
• Gene ID (NCBI): 3155
• RRID: AB_2295304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35914
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	WB,IHC	Hepatol Int; HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility; Authors - Xiaohan Cui
mouse	WB	J Biol Chem; Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation; Authors - Emily L Goldberg
human,mouse	WB,IHC,IP	Int J Biol Sci; Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer; Authors - Chenxi Zhong ; KD Validated
mouse	WB	Cell Rep; Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria; Authors - Sarah L Speck