HMGCL Polyclonal antibody

HMGCL Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16898-1-AP

Synonyms

HL, HMG CoA lyase, HMGCL



Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman liver tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16898-1-AP targets HMGCL in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGCL fusion protein Ag10426
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC010570
Gene Symbol HMGCL
Gene ID (NCBI) 3155
RRIDAB_2295304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HMGCL antibody 16898-1-APDownload protocol
IHC protocol for HMGCL antibody 16898-1-APDownload protocol
IP protocol for HMGCL antibody 16898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Cell Biol

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Authors - Jin-Tao Li
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
humanWB,IHC

Hepatol Int

HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility

Authors - Xiaohan Cui
mouseWB

J Biol Chem

Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation

Authors - Emily L Goldberg
human,mouseWB,IHC,IP

Int J Biol Sci

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer

Authors - Chenxi Zhong
  • KD Validated
mouseWB

Cell Rep

Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria

Authors - Sarah L Speck