HMGCLL1 Polyclonal antibody

HMGCLL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17206-1-AP

Synonyms

HMGCLL1

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Validation Data Gallery

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  WB analysis of SKOV-3 using 17206-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17206-1-AP (HMGCLL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 17206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: SKOV-3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17206-1-AP is HMGCLL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGCLL1 fusion protein Ag10985
• Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
• Calculated Molecular Weight: 340 aa, 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC024194
• Gene Symbol: HMGCLL1
• Gene ID (NCBI): 54511
• RRID: AB_2878362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa.