HNF1B Polyclonal antibody, PBS Only

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• HNF1B Polyclonal antibody, PBS Only (12533-1-PBS)

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  WB analysis of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 12533-1-AP (same clone as 12533-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12533-1-AP (same clone as 12533-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12533-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

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Product Information

12533-1-PBS targets HNF1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3232

  Product name: Recombinant human HNF1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC017714

  Sequence: MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFR

• Full Name: HNF1 homeobox B
• Calculated Molecular Weight: 557 aa, 61 kDa
• Observed Molecular Weight: 58-65 kDa
• GenBank Accession Number: BC017714
• Gene Symbol: HNF1B
• Gene ID (NCBI): 6928
• RRID: AB_2116758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35680
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.