CoraLite® Plus 488-conjugated HNRNPA1 Monoclonal antibody

HNRNPA1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5A4

Cat no : CL488-67844

Synonyms

Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67844

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HNRNPA1 antibody (CL488-67844, Clone: 1A5A4 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67844 targets HNRNPA1 in IF applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HNRNPA1 fusion protein Ag17415
• Full Name: heterogeneous nuclear ribonucleoprotein A1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009600
• Gene Symbol: HNRNPA1
• Gene ID (NCBI): 3178
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.