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HNRNPC Recombinant antibody

HNRNPC Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F16

Cat no : 82798-3-RR

Synonyms

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPC Recombinant antibody (82798-3-RR)

    WB analysis using 82798-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82798-3-RR (HNRNPC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPC Recombinant antibody (82798-3-RR)

    IF Staining of HeLa using 82798-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (82798-3-RR, Clone: 4F16 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). CoraLite® Plus 488-Phalloidin (green).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82798-3-RR targets HNRNPC in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPC fusion protein Ag2356
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC003394
Gene Symbol HNRNPC
Gene ID (NCBI) 3183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 82798-3-RRDownload protocol
IF protocol for HNRNPC antibody 82798-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols