Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67375-1-Ig targets HNRNPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6603 Product name: Recombinant human HNRNPH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 104-449 aa of BC001348 Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein H1 (H) |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49-50 kDa |
| GenBank Accession Number | BC001348 |
| Gene Symbol | HNRNPH1 |
| Gene ID (NCBI) | 3187 |
| RRID | AB_2882623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31943 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| IHC protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| WB protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (Cat# 67375-1-Ig) has 4 total citations, published in International Journal of Biological Macromolecules, Cell Proliferation, Journal of Cellular Physiology, and Frontiers in Oncology. Research fields span viral replication (Japanese encephalitis virus), Crohn's disease with macrophage–myofibroblast transition, lung adenocarcinoma RNA splicing, and Ewing sarcoma chromatin accessibility. Applications include WB and IF across human and mouse species.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Japanese encephalitis virus NS3 captures the protein translation element by interacting with HNRNPH1 to promote viral replication
| ||
Cell Prolif AMSC-sEVs Ameliorated Crohn's Disease by Inhibiting Macrophage-Myofibroblast Transition Through the Delivery of MFGE8. | ||
J Cell Physiol MACC1 enhances an oncogenic RNA splicing of IRAK1 through interacting with HNRNPH1 in lung adenocarcinoma | ||
Front Oncol MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing |
