HNRNPH1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 67375-1-Ig (same clone as 67375-1-PBS)

  WB result of HNRNPH1 antibody (67375-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPH1 transfected HEK-293T cells. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51734-1

  Cytometric bead array sample test of MP51734-1, HNRNPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67375-1-PBS. Detection antibody: 67375-2-PBS.

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  Cytometric bead array standard curve of MP51734-1

  Cytometric bead array standard curve of MP51734-1, HNRNPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67375-1-PBS. Detection antibody: 67375-2-PBS. Standard:Ag6603. Range: 3.125-400 ng/mL.

• Featured Product
• KD/KO Validated

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67375-1-PBS

Clone No.1A6C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A6C2, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH

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Product Information

67375-1-PBS targets HNRNPH1 as part of a matched antibody pair:

MP51734-1: 67375-1-PBS capture and 67375-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6603

  Product name: Recombinant human HNRNPH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 104-449 aa of BC001348

  Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

• Full Name: heterogeneous nuclear ribonucleoprotein H1 (H)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-50 kDa
• GenBank Accession Number: BC001348
• Gene Symbol: HNRNPH1
• Gene ID (NCBI): 3187
• RRID: AB_2882623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P31943
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].