CoraLite® Plus 488-conjugated HNRNPR Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-85078-5

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HNRNPR antibody (CL488-85078-5, Clone: 242734A8 ) at dilution of 1:200, CL594-Phalloidin (red).

HNRNPR Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85078-5

Clone No.242734A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

hnRNP R, HNRPR

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85078-5 targets HNRNPR in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30339

  Product name: Recombinant human HNRNPR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 423-475 aa of BC001449

  Sequence: QAARQASRSTAYEDYYYHPPPRMPPPIRGRGRGGGRGGYGYPPDYYGYEDYYD

• Full Name: heterogeneous nuclear ribonucleoprotein R
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 65-82 kDa
• GenBank Accession Number: BC001449
• Gene Symbol: HNRNPR
• Gene ID (NCBI): 10236
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43390
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)