HNRNPU Recombinant monoclonal antibody

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• HNRNPU Recombinant monoclonal antibody (84964-4-RR)

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  WB analysis using 84964-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 84964-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 84964-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84964-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (84964-4-RR, Clone: 242609C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84964-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

HNRNPU Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 84964-4-RR

Clone No.242609C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242609C9, C1orf199, GRIP120, hnRNP U, HNRPU

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells,
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84964-4-RR targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6141

  Product name: Recombinant human HNRNPU protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 10-236 aa of BC003621

  Sequence: KLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRSGAGLEQEAAAGGDEEEEEEEEEEEGISALDGDQMELGEENGAAGAADSGPMEEEEAASEDENGDDQGFQEGEDELGDEEEGAGDENGHGEQQPQPPATQQQQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTEQKGGDKKRGVKRPREDHGR

• Full Name: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• Calculated Molecular Weight: 825 aa, 91 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC003621
• Gene Symbol: HNRNPU
• Gene ID (NCBI): 3192
• RRID: AB_3743891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00839
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).