HO-1/HMOX1 Polyclonal antibody, PBS Only

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• HO-1/HMOX1 Polyclonal antibody, PBS Only (10701-1-PBS)

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  WB analysis of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

  WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  WB analysis using 10701-1-AP (same clone as 10701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  WB analysis using 10701-1-AP (same clone as 10701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

  IP result of anti-HO-1/HMOX1 (IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

  Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HO-1/HMOX1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA

Cat No. 10701-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA

Synonyms

HMOX1, HO-1, bK286B10, EC:1.14.14.18, heme oxygenase (decycling) 1

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Product Information

10701-1-PBS targets HO-1/HMOX1 in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1190

  Product name: Recombinant human HO-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-288 aa of BC001491

  Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM

• Full Name: heme oxygenase (decycling) 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 28-33 kDa
• GenBank Accession Number: BC001491
• Gene Symbol: HO-1
• Gene ID (NCBI): 3162
• RRID: AB_2118685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09601
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).