Haptoglobin Monoclonal antibody

Haptoglobin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B4B9

Cat no : 66229-1-Ig

Synonyms

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

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Validation Data Gallery

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  WB analysis of human plasma using 66229-1-Ig

  0.5 μl human plasma was subjected to SDS PAGE followed by western blot with 66229-1-Ig (HP Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66229-1-Ig targets Haptoglobin in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: monkey
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Haptoglobin fusion protein Ag9927
• Full Name: haptoglobin
• Calculated Molecular Weight: 281aa,31 kDa; 228aa,25 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC058031
• Gene Symbol: HP
• Gene ID (NCBI): 3240
• RRID: AB_2881620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

Publications

Species	Application	Title
monkey	WB	Int J Radiat Oncol Biol Phys; Identification of Novel Biomarkers for Acute Radiation Injury Using Multiomics Approach and Nonhuman Primate Model; Authors - Amrita K Cheema