HPDL Polyclonal antibody

HPDL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20777-1-AP

Synonyms

HPD-like protein, Glyoxalase domain-containing protein 1, GLOXD1, EC:1.13.-.-, 4-hydroxyphenylpyruvate dioxygenase-like protein

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Validation Data Gallery

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  WB analysis using 20777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20777-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20777-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20777-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 20777-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 20777-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 20777-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of Caco-2 using 20777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HPDL antibody (20777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: human stomach tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPDL fusion protein Ag14773
• Full Name: 4-hydroxyphenylpyruvate dioxygenase-like
• Calculated Molecular Weight: 371 aa, 39 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC007293
• Gene Symbol: HPDL
• Gene ID (NCBI): 84842
• RRID: AB_10697807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.