Tested Applications
| Positive WB detected in | HepG2 cells, Caco-2 cells, L02 cells |
| Positive IHC detected in | human liver tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14773 Product name: Recombinant human HPDL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-371 aa of BC007293 Sequence: MAAPALRLCHIAFHVPAGQPLARNLQRLFGFQPLASREVDGWRQLALRSGDAVFLVNEGAGSGEPLYGLDPRHAVPSATNLCFDVADAGAATRELAALGCSVPVPPVRVRDAQGAATYAVVSSPAGILSLTLLERAGYRGPFLPGFRPVSSAPGPGWVSRVDHLTLACTPGSSPTLLRWFHDCLGFCHLPLSPGEDPELGLEMTAGFGLGGLRLTALQAQPGSIVPTLVLAESLPGATTRQDQVEQFLARHKGPGLQHVGLYTPNIVEATEGVATAGGQFLAPPGAYYQQPGKERQIRAAGHEPHLLARQGILLDGDKGKFLLQVFTKSLFTEDTFFLELIQRQGATGFGQGNIRALWQSVQEQSARSQEA Predict reactive species |
| Full Name | 4-hydroxyphenylpyruvate dioxygenase-like |
| Calculated Molecular Weight | 371 aa, 39 kDa |
| Observed Molecular Weight | 35-39 kDa |
| GenBank Accession Number | BC007293 |
| Gene Symbol | HPDL |
| Gene ID (NCBI) | 84842 |
| RRID | AB_10697807 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96IR7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HPDL antibody 20777-1-AP | Download protocol |
| IHC protocol for HPDL antibody 20777-1-AP | Download protocol |
| WB protocol for HPDL antibody 20777-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
20777-1-AP (anti-HPDL antibody) has a total of 2 citations. The journals are Oncology Research and Gene. The associated research fields include oncology (histone emulsification and EC cell proliferation) and neurology/genetics (neurodevelopmental disorder with progressive spasticity and brain white matter abnormalities).
| Species | Application | Title |
|---|---|---|
Oncol Res Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation.
| ||
Gene Two novel heterozygous HPDL variants in a Chinese family with a neurodevelopmental disorder with progressive spasticity and brain white matter abnormalities |
Reviews
What customers say
The antibody received a 4/5 rating in a Western Blot application using an Ewing Sarcoma Cell Line at a 1:500 dilution. The reviewer confirmed the antibody's specificity by performing a knockdown experiment with a SMARTpool siRNA targeting HPDL, observing a corresponding decrease in protein signal at the expected molecular weight. Overall, the product demonstrated reliable specificity and performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Taras (Verified Customer) (10-21-2025) | Specificity of the antibody was tested using a SMARTpool siRNA against HPDL with a decrease of the protein at expected molecular weight
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