HPS5 Polyclonal antibody

HPS5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13901-1-AP

Synonyms

AIBP63, Hermansky Pudlak syndrome 5, HPS5, KIAA1017, Ru2, Ruby eye protein 2 homolog

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Validation Data Gallery

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  WB analysis of mouse liver using 13901-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13901-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 13901-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HPS5 antibody 13901-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse spleen tissue
• Positive IHC detected in: human placenta tissue, human heart tissue, human lung tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

13901-1-AP targets HPS5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPS5 fusion protein Ag4867
• Full Name: Hermansky-Pudlak syndrome 5
• Calculated Molecular Weight: 1129 aa, 127 kDa
• Observed Molecular Weight: 135-141 kDa
• GenBank Accession Number: BC033640
• Gene Symbol: HPS5
• Gene ID (NCBI): 11234
• RRID: AB_2120685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hermansky-Pudlak syndrome 5 protein (HPS-5), also named Ruby-eye protein 2 homolog (Ru2), plays a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5.

Publications

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
human	WB	Am J Hum Genet; A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.; Authors - Cullinane Andrew R AR
human	WB	J Invest Dermatol; Clinical, molecular, and cellular features of non-Puerto Rican Hermansky-Pudlak syndrome patients of Hispanic descent.; Authors - Carmona-Rivera Carmelo C
human	WB	Pigment Cell Melanoma Res; A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.; Authors - Cullinane Andrew R AR
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen
human	WB	Front Genet; Oculocutaneous albinism and bleeding diathesis due to a novel deletion in the HPS3 gene; Authors - Dina Marek-Yagel