• Featured Product
  • KD/KO Validated

HRAS-Specific Polyclonal antibody

HRAS-Specific Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18295-1-AP

Synonyms

C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, HRAS-Specific, K RAS, RASH1, Transforming protein p21



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat tissue
Positive IP detected inmouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

18295-1-AP targets HRAS-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC006499
Gene symbol HRAS
Gene ID (NCBI) 3265
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Protocols

Product Specific Protocols
WB protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IHC protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IP protocol for HRAS-Specific antibody 18295-1-APDownload protocol
FC protocol for HRAS-Specific antibody 18295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.

Authors - Sarah J Ross
humanWB

Sci Transl Med

The ERBB network facilitates KRAS-driven lung tumorigenesis.

Authors - Björn Kruspig
humanIP

Mol Cell

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis

Authors - Hiroshi Senoo
humanIF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che
humanWB

Nat Commun

A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.

Authors - Nicolas Bery
humanWB

Clin Cancer Res

Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.

Authors - Chen Liu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK